Correlated Noise in a Synthetic Gene Circuit Reveals Regulation
ثبت نشده
چکیده
Simulations and analysis in the previous chapter are based on parameters for the λ cI system. Here, we test those predictions experimentally by building a synthetic circuit using the λ cI repressor and other characterized circuit elements in E. coli bacteria. The synthetic circuit uses three fluorescent proteins so that noisy gene expression can be monitored with time-lapse microscopy. To apply the analysis methods in the previous chapter, the cross correlation calculation is extended to allow for branched data where all progeny originate from a single ancestor. Experimental cross correlation curves show features that were predicted in simulation and results are tested further by comparing two different noise backgrounds to study the relative effect of extrinsic and intrinsic noise.
منابع مشابه
Noise propagation in synthetic gene circuits for metabolic control.
Dynamic control of enzyme expression can be an effective strategy to engineer robust metabolic pathways. It allows a synthetic pathway to self-regulate in response to changes in bioreactor conditions or the metabolic state of the host. The implementation of this regulatory strategy requires gene circuits that couple metabolic signals with the genetic machinery, which is known to be noisy and on...
متن کاملLow Dropout Based Noise Minimization of Active Mode Power Gated Circuit
Power gating technique reduces leakage power in the circuit. However, power gating leads to large voltage fluctuation on the power rail during power gating mode to active mode due to the package inductance in the Printed Circuit Board. This voltage fluctuation may cause unwanted transitions in neighboring circuits. In this work, a power gating architecture is developed for minimizing power in a...
متن کاملAutomatic Design of Digital Synthetic Gene Circuits
De novo computational design of synthetic gene circuits that achieve well-defined target functions is a hard task. Existing, brute-force approaches run optimization algorithms on the structure and on the kinetic parameter values of the network. However, more direct rational methods for automatic circuit design are lacking. Focusing on digital synthetic gene circuits, we developed a methodology ...
متن کاملA New Circuit Scheme for Wide Dynamic Circuits
In this paper, a new circuit scheme is proposed to reduce the power consumption of dynamic circuits. In the proposed circuit, an NMOS keeper transistor is used to maintain the voltage level in the output node against charge sharing, leakage current and noise sources. Using the proposed keeper scheme, the voltage swing on the dynamic node is lowered to reduce the power consumption of wide fan-in...
متن کاملA synthetic three-color scaffold for monitoring genetic regulation and noise
BACKGROUND Current methods for analyzing the dynamics of natural regulatory networks, and quantifying synthetic circuit function, are limited by the lack of well-characterized genetic measurement tools. Fluorescent reporters have been used to measure dynamic gene expression, but recent attempts to monitor multiple genes simultaneously in single cells have not focused on independent, isolated me...
متن کامل